HER2 expression in breast cancer is critical for targeting, and the survival of HER2-positive patients have significantly improved with targeted antibodies. Traditional methods for analyzing HER2 expression in breast cancer are post-biopsy following immunohistochemistry and fluorescence in situ hybridization, which are characterized by invasiveness, incorrect presentation of HER2 expression in sampled specimens, and difficulty in sampling distant metastases. It is imperative to improve the expression analysis of HER2 in breast cancer, among which radio-labeled HER2 probes are a good choice. The HER2 affibody probe of breast cancer has been proved to have high affinity and imaging performance in clinical trials.